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IBB - Institute for Biotechnology and Bioengineering
CEB - Centre of Biological Engineering - University of Minho		 Universidade do Minho - Escola de Engenharia
 

 

CEB is part of the Institute for Biotechnology and Bioengineering

 
Nuno Miguel Dias Cerca
Preferred Name:
Nuno Cerca
E-Mail Address:
nunocerca@ceb.uminho.pt @deb.uminho.pt
Activity:
Investigador Auxiliar
Project / Theme:
Transcriptomics and Proteomics
Start Date: 6/2/2008
End Date: 5/31/2013
Faculty Supervisor: Nuno Cerca
Telephone: Office:604423
Laboratory: 601970
Laboratory: Plataforma de Biologia Molecular e Sintética
Office: Gabinete de Investigadores CIENCIA
Birthday: April 23th
Website: http://nunocerca.wordpress.com
Access after working hours: Yes
Nationality: Portugal
Last Update: 8/2/2011

Selected Publications

1. Cerca, N.
  Biofilm transcriptomics handbook: Quantifying gene expression from pathogenic bacterial biofilms.
  Biofilm transcriptomics handbook: Quantifying gene expression from pathogenic bacterial biofilms (), -, 2011.
 
2. Melo, L.; Sillankorva, S.; Azeredo, J.; Cerca, N.
  Efficacy studies of a S. epidermidis bacteriophage against stationary and biofilm cells .
  MicroBiotec'11 (), -, 2011.
 
3. Cerca, F.; França, A.; Guimaraes, R.; Hinzmann, M.; Cerca, N.; Lobo da Cunha, A.; Azeredo, J.; Vilanova, M.
  Modulation of poly-N-acetylglucosamine accumulation within mature Staphylococcus epidermidis biofilms grown in excess glucose.
  Microbiology and Immunology 10(55), 673-682, 2011.
 
4. Cerca, F.; Branquinho, G.; Correia, A.; Cerca, N.; Azeredo, J.; Vilanova, M.
  SYBR green as a fluorescent probe to evaluate the biofilm physiological state of Staphylococcus epidermidis, using flow cytometry.
  Canadian Journal of Microbiology 57(10), 850-856, 2011.
 
5. Gomes, F.I.; Teixeira, P.; Cerca, N.; Ceri, H.; Oliveira, R.
  Virulence gene expression by Staphylococcus epidermidis biofilm cells exposed to antibiotics.
  Microbial Drug Resistance 2(17), 191-196, 2011.
 
6. Gomes, F.I.; Teixeira, P.; Cerca, N.; Azeredo, J.; Oliveira, R.
  Effect of farnesol on structure and composition of Staphylococcus epidermidis biofilm matrix .
  Current Microbiology 63(4), 354-359, 2011.
 
7. Freitas, A.I.; Henriques, A.; Cerca, N.
  Getting the best out of your gene expression assay: an optimized protocol.
  Biofilm transcriptomics handbook: Quantifying gene expression from pathogenic bacterial biofilms (), 195-211, 2011.
 
8. França, A.; Bento, J.; Cerca, N.
  Comparative study of gene expression in gram-positive and gram-negative biofilms.
  Biofilm transcriptomics handbook: Quantifying gene expression from pathogenic bacterial biofilms (), 175-193, 2011.
 
9. França, A.; Cerca, N.
  Methodology used for real-time PCR results analysis.
  Biofilm transcriptomics handbook: Quantifying gene expression from pathogenic bacterial biofilms (), 145-154, 2011.
 
10. Melo, L.; Cerca, N.
  Primer design using freeware available of the web.
  Biofilm transcriptomics handbook: Quantifying gene expression from pathogenic bacterial biofilms (), 133-144, 2011.
 
11. Bento, J.; Carvalhais, V.; Cerca, N.
  Overview of polymerase chain reaction and key concepts of real time PCR.
  Biofilm transcriptomics handbook: Quantifying gene expression from pathogenic bacterial biofilms (), 115-131, 2011.
 
12. Machado, A.; Cerca, N.; Jefferson, K.
  Gardenerella vaginalis: the role of biofilms in the pathogenesis of bacterial vaginosis.
  Biofilm transcriptomics handbook: Quantifying gene expression from pathogenic bacterial biofilms (), 41-51, 2011.
 
13. Melo, L.; França, A.; Cerca, N.
  Staphylococcus epidermidis and biofilm infections in indwelling medical devices.
  Biofilm transcriptomics handbook: Quantifying gene expression from pathogenic bacterial biofilms (), 29-40, 2011.
 
14. Cerca, N.
  Introduction to Biofilms.
  Biofilm transcriptomics handbook: Quantifying gene expression from pathogenic bacterial biofilms (), 13-27, 2011.
 
15. Freitas, A.I.; França, A.; Vasconcelos, C; Vilanova, M.; Cerca, N.
  Cell-to-cell aggregation in S. epidermidis and its effect on quantification of total and viable bacteria within biofilms .
  BioMicroWorld2011 - IV International Conference on Environmental, Industrial and Applied Microbiology (), -, 2011.
 
16. França, A.; Vilanova, M.; Cerca, N.
  High variability of gene expression in S. epidermidis biofilm population.
  BioMicroWorld2011 - IV International Conference on Environmental, Industrial and Applied Microbiology (), -, 2011.
 
17. Melo, L.; Cece, E.; Sillankorva, S.; Azeredo, J.; Cerca, N.
  An urgent seek for S. epidermidis large host range bacteriophages.
  19th Biannual Evergreen International Phage Biology Meeting (), -, 2011.
 
18. Machado, A.; Carvalho, A.; Almeida, C.; Salgueiro, D.; Vieira, M.J.; Rodrigues, L.R.; Azevedo, N.F.; Cerca, N.; Henriques, A.
  Comparison between classical and molecular (FISH and PCR) methods for Lactobacillus spp. detection in Clinical Samples.
  Gordon Research Conference on Microbial Adhesion & Signal Transductio (), -, 2011.
 
19. França, A.; Bento, J.; Pier, G.; Vilanova, M.; Cerca, N.
  Comparison of RNA extraction methods from biofilm samples of Staphylococcus epidermidis and other bacterial pathogens.
  FEMS 4th Congress of European Microbiologists (), -, 2011.
 
20. Machado, A.; Carvalho, A.; Almeida, C.; Vieira, M.J.; Rodrigues, L.R.; Cerca, N.; Azevedo, N.F.
  Lactobacillus spp. Identification in Mixed Samples by PNA FISH.
  Eurobiofilms 2011 (), -, 2011.
 
21. Rodrigues, D.; Cerca, N.; Teixeira, P.; Oliveira, R.; Ceri, H.; Azeredo, J.
  Listeria monocytogenes and Salmonella enterica Enteritidis biofilms susceptibility to different disinfectants and stress-response and virulence gene expression of surviving cells.
  Microbial Drug Resistance 17(2), 181-189, 2011.
 
22. Gomes, F.I.; Teixeira, P.; Cerca, N.; Azeredo, J.; Oliveira, R.
  The comparative effect of farnesol and antibiotics against Staphylococcus epidermidis.
  Biofilms 2009 - 5th ASM Conference on Biofilms (B47), 52-, 2009.
 
23. Cerca, N.; Pier, G.B.; Oliveira, R.; Azeredo, J.
  Adhesion and biofilm formation of clinical isolates of Staphylococcus epidermidis.
  Gordon Conference for Staphylococcal Diseases (), -, 2003.
 
24. Cerca, N.; Martins, S.; Pier, G.; Oliveira, R.; Azeredo, J.
  Can studies of inhibition of adhesion by sub-mic concentrations of antibiotics predict the outcome in biofilm formation inhibition?.
  11th Annual International Symposium on Staphylococci & Staphylococcal Infections (), -, 2004.
 
25. Cerca, N.; Martins, S.; Cerca, F.; Pier, G.B.; Oliveira, R.; Azeredo, J.
  Resistance of coagulase-negative Staphyloccocci biofilms to antibiotics.
  VII International Meeting on Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases (MEEGID VII) (), -, 2004.
 
26. Cerca, N.; Jefferson, K.; Grout, M.; Goldmann, D.; Azeredo, J.; Oliveira, R.
  Immunological Similarities Between Poly-N-acetyl Glucosamine (PNAG) from Staphylococcus spp. and Escherichia coli.
  ASM 105th General Meeting (), -, 2005.
 
27. Cerca, F.; Andrade, F.; Cerca, N.; Lobo-da-Cunha, A.; Azeredo, J.; Vilanova, M.
  Bacterial population heterogeneity and extracellular matrix production in Staphylococcus epidermidis biofilms.
  Book of Abstracts of EUROBIOFILMS 2009 - First European Congress on Microbial Biofilms (P184), 170-171, 2009.
 
28. Gomes, F.I.; Cerca, N.; Ceri, H.; Teixeira, P.; Oliveira, R.
  Effect of single versus antibiotic combinations on Staphylococcus epidermidis biofilm viability and genetic expression of some virulence genes.
  Conference Handbook of the 4th International Conference Biofilms IV: Communities Bridging Disciplines (118), 71-, 2010.
 
29. Gomes, F.I.; Leite, B.; Teixeira, P.; Cerca, N.; Azeredo, J.; Oliveira, R.
  Farnesol as antibiotics adjuvant in Staphylococcus epidermidis control in vitro.
  American Journal of the Medical Sciences 341(3), 191-195, 2011.
 
30. Gomes, F.I.; Cerca, N.; Teixeira, P.; Oliveira, R.
  Antimicrobial activity of farnesol against Staphylococcus epidermidis.
  Book of Abstracts of EUROBIOFILMS 2009 - First European Congress on Microbial Biofilms (), 83-84, 2009.
 
31. Cerca, N.; Maira-Litrán, T.; Jefferson, K.K.; Grout, M.; Goldmann, D.A.; Pier, G.B.
  Protection against Escherichia coli infection by antibody to the Staphylococcus aureus poly-N-acetylglucosamine surface polysaccharide.
  Proceedings of the National Academy of Sciences of the United States of America 104(18), 7528-7533, 2007.
 
32. Cerca, N.; Oliveira, R.; Azeredo, J.
  Susceptibility of staphylococcus epidermidis planktonic cells and biofilms to the lytic action of staphylococcus bacteriophage K.
  Letters in Applied Microbiology 45(3), 313-317, 2007.
 
33. Cerca, N.; Jefferson, K.K.; Maira-Litrán, T.; Pier, D.B.; Kelly-Quintos, C.; Goldmann, D.A.; Azeredo, J.; Pier, G.B.
  Molecular basis for preferential protective efficacy of antibodies directed to the poorly acetylated form of staphylococcal poly-N-acetyl-β-(1-6)-glucosamine.
  Infection and Immunity 75(7), 3406-3413, 2007.
 
34. Cerca, N.
  Virulence aspects of Staphylococcus epidermidis: biofilm formations and Poly-N-Acetyl-Glucosamine production.
  Doutoramento em Engenharia Química e Biológica (), -, 2006.
 
35. Cerca, N.; Jefferson, K.K.; Oliveira, R.; Pier, G.B.; Azeredo, J.
  Comparative antibody-mediated phagocytosis of Staphylococcus epidermidis cells grown in a biofilm or in the planktonic state.
  Infection and Immunity 74(8), 4849-4855, 2006.
 
36. Cerca, N.; Pier, G.B.; Jefferson, K.K.; Oliveira, R.; Azeredo, J.
  Use of confocal laser scan microscopy (CLSM) to evaluate S. epidermidis biofilm structure: the effect of low concentration of antibiotics - a case study.
  Proceedings of the International Conference BIOFILMS II: attachment and detachment in pure and mixed cultures (), -, 2006.
 
37. Cerca, N.; Martins, S.; Sillankorva, S.; Pier, Gerald B.; Oliveira, R.; Azeredo, J.
  Changes in CoNS biofilm formation, composition, structure and antimicrobial resistance due to growth in sub-inhibitory concentrations of dicloxacillin.
  Proceedings of the International Conference Biofilms 2004: Structure and Activity of Biofilms - Poster Presentations 2004(), 110-114, 2004.
 
38. Cerca, N.; Pier, Gerald B.; Oliveira, R.; Azeredo, J.
  Inhibition of adherence of coagulase-negative Staphylococci (CoNS) to acrylic by subinhibitory concentrations of antibiotics.
  Proceedings of the 1st European Conference on Biofilms – Prevention of Microbial Adhesion (), 43-, 2004.
 
39. Cerca, N.; Pier, Gerald B.; Oliveira, R.; Azeredo, J.
  Differences in adhesion and biofilm formation of several clinical isolates of Staphylococcus epidermidis.
  Biofilms 2003 - 3rd ASM Conference on Biofilms (318(C)), 163-, 2003.
 
40. Henriques, M.; Cerca, N.; Azeredo, J.; Oliveira, R.
  Influence of sub-inhibitory concentrations of antimicrobial agents on biofilm formation in indwelling medical devices.
  International Journal of Artificial Organs 28(11), 1181-1185, 2005.
 
41. Cerca, N.; Pier, Gerald B.; Oliveira, R.; Azeredo, J.; Jefferson, Kimberly K.
  Effect of a single locus deletion on global genome expression: the ica locus in Staphyloccocus spp: a case study.
  Micro’05-Biotec’05: Livro de Actas do Congresso MICROBIOTEC’2005 (397), -, 2005.
 
42. Cerca, Filipe; Teixeira, Luzia; Cerca, N.; Madureira, Pedro; Ribeiro, Adília; Azeredo, J.; Vilanova, Manuel
  A study on the immune response elicited in mice challenged with Staphylococcus epidermidis planktonic - or biofilm - grown cells.
  Micro’05-Biotec’05: Livro de Actas do Congresso MICROBIOTEC’2005 338.(), -, 2005.
 
43. Cerca, N.; Cerca, Filipe; Martins, S.; Pier, Gerald B.; Oliveira, R.; Azeredo, J.
  Synergistic effects of combinations of antibiotics on coagulase negative staphylococcal (CoNS) biofilms.
  Abstracts of the XI International Congress of Mycology IUMS 2005 - Microbes in a Changing World (), 9-, 2005.
 
44. Cerca, N.; Azeredo, J.; Oliveira, R.; Pier, Gerald B.; Jefferson, Kimberly K.
  Environmental regulation of the pgaABCD locus in Escherichia coli.
  Abstracts of the XI International Congress of Mycology IUMS 2005 - Microbes in a Changing World (), 9-, 2005.
 
45. Cerca, N.; Martins, S.; Cerca, F.; Jefferson, K.K.; Pier, G.B.; Oliveira, R.; Azeredo, J.
  Comparative assessment of antibiotic susceptibility of coagulase-negative staphylococci in biofilm versus planktonic culture as assessed by bacterial enumeration or rapid XTT colorimetry.
  Journal of Antimicrobial Chemotherapy 56(2), 331-336, 2005.
 
46. Cerca, N.; Martins, S.; Sillankorva, S.; Jefferson, K.K.; Pier, G.B.; Oliveira, R.; Azeredo, J.
  Effects of growth in the presence of subinhibitory concentrations of dicloxacillin on Staphylococcus epidermidis and Staphylococcus haemolyticus biofilms.
  Applied and Environmental Microbiology 71(12), 8677-8682, 2005.
 
47. Cerca, N.; Martins, S.; Pier, G.B.; Oliveira, R.; Azeredo, J.
  The relationship between inhibition of bacterial adhesion to a solid surface by sub-MICs of antibiotics and subsequent development of a biofilm.
  Research in Microbiology 156(5-6), 650-655, 2005.
 
48. Cerca, N.; Pier, G.B.; Vilanova, M.; Oliveira, R.; Azeredo, J.
  Quantitative analysis of adhesion and biofilm formation on hydrophilic and hydrophobic surfaces of clinical isolates of Staphylococcus epidermidis.
  Research in Microbiology 156(4), 506-514, 2005.
 
49. Cerca, N.; Pier, G.B.; Vilanova, M.; Oliveira, R.; Azeredo, J.
  Influence of batch or fed-batch growth on Staphylococcus epidermidis biofilm formation.
  Letters in Applied Microbiology 39(), 420-424, 2004.
 
50. Cerca, N.; Pier, G.B.; Oliveira, R.; Azeredo, J.
  Comparative evaluation of coagulase-negative staphylococci (CoNS) adherence to acrylic by a static method and a parallel-plate flow dynamic method.
  Research in Microbiology 155(9), 755-760, 2004.