Biofilm transcriptomics: Quantifying gene expression from pathogenic bacterial biofilms


 In this intensive practical workshop the participants will be able to learn how to work with complex samples and how to quantify the genetic expression of genes of interest.

Planktonic free-floating bacteria express different phenotypes as compared with bacteria inside the biofilm communities.  Studying  these differences is of the utmost importance in order to better understand why bacteria in biofilms are normally more pathogenic than planktonic bacteria.  Analysing the genetic expression of such bacterial communities provides some challenges mainly due to the presence of extracellular polymeric substances surrounding the bacteria, what is called the biofilm matrix. In this intensive practical workshop the participants will be able to learn how to work with complex samples and how to quantify the genetic expression of genes of interest.



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