Publications in International Journals / Series (Article (International))

Isolation and purification of an enzyme hydrolyzing ochratoxin A from Aspergillus niger

  • Biotechnology Letters, 29(12), 1909-1914, 2007

Publisher: Springer Nature

Abstract

Ochratoxin A is a mycotoxin produced by several Aspergillus and some Penicillium species which may be present in food and feed products. It can be enzymatically hydrolyzed into ochratoxin α and l-β-phenylalanine, thereby decreasing its toxicity. The ochratoxin A degradation capacity of Aspergillus niger is well known and here we report the isolation and purification of a novel enzyme from A. niger that hydrolyzes this mycotoxin. A wheat germ medium supplemented with ochratoxin A was used to produce the enzyme, which was purified from culture filtrate by acetone precipitation and anion exchange ...

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