Publications in International Journals / Series (Article (International))

Assessment of liposome disruption to quantify drug delivery in vitro

  • Biochimica et Biophysica Acta-Biomembranes, 1858(2), 163-167, 2016

Publisher: Elsevier

Abstract

Efficient liposome disruption inside the cells is a key for success with any type of drug delivery system. The efficacy of drug delivery is currently evaluated by direct visualization of labeled liposomes internalized by cells, not addressing objectively the release and distribution of the drug. Here, we propose a novel method to easily assess liposome disruption and drug release into the cytoplasm. We propose the encapsulation of the cationic dye Hoechst 34,580 to detect an increase in blue fluorescence due to its specific binding to negatively charged DNA. For that, the dye needs to be relea ...

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