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Strategies to suppress hydrogen-consuming microorganisms affect macro and micro scale structure and microbiology of granular sludge

  • Biotechnology and Bioengineering, 108(8), 1766-1775, 2011

Publisher: Wiley-Blackwell

Abstract

Treatment of anaerobic granules with heat and two chemical treatments, contacting with 2-bromoethanesulfonate (BES) and with BES+Chloroform, were applied to suppress hydrogen-consuming microorganisms. Three mesophilic expanded granular sludge bed (EGSB) reactors - RHeat, RBES and RBES+Chlo - were inoculated with the treated sludges and fed with synthetic sugar-based wastewater (5 gCODL−1, HRT 20-12h). Morphological integrity of granules and bacterial communities were assessed by quantitative image analysis and 16S rRNA gene based techniques, respectively. Hydrogen production in RHeat was under ...

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