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Power analysis of single-cell RNA-sequencing experiments

  • Svensson, Valentine; Natarajan, Kedar Nath; Ly, Lam-Ha; Miragaia, Ricardo J.; Labalette, Charlotte; Macaulay, Iain C.; Cvejic, Ana; Teichmann, Sarah A.
  • Nature Methods, (In Press), 2017

Publisher: Springer Nature

Abstract

Single-cell RNA sequencing (scRNA-seq) has become an established and powerful method to investigate transcriptomic cell-to-cell variation, thereby revealing new cell types and providing insights into developmental processes and transcriptional stochasticity. A key question is how the variety of available protocols compare in terms of their ability to detect and accurately quantify gene expression. Here, we assessed the protocol sensitivity and accuracy of many published data sets, on the basis of spike-in standards and uniform data processing. For our workflow, we developed a flexible tool for ...

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