Publications in International Journals / Series (Article (International))

Toxic reagents and expensive equipment: are they really necessary for the extraction of good quality fungal DNA?

  • Letters in Applied Microbiology, 66(1), 32-37, 2018

Publisher: Wiley-Blackwell


The aim of this work was to evaluate a fungal DNA extraction procedure with the lowest inputs in terms of time as well as of expensive and toxic chemicals, but able to consistently produce genomic DNA of good quality for PCR purposes. Two types of fungal biological material were tested - mycelium and conidia - combined with two protocols for DNA extraction using Sodium Dodecyl Sulphate [SDS] and Cetyl Trimethyl Ammonium Bromide [CTAB] as extraction buffers and glass beads for mechanical disruption of cell walls. Our results showed that conidia and SDS buffer was the combination that lead to th ...

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