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Effects of gamma irradiation and periodate oxidation on the structure of dextrin assessed by mass spectrometry

  • European Polymer Journal, 103, 158-169, 2018

Publisher: Elsevier

Abstract

Sterilization of biomaterials by gamma irradiation should not change its structure, since this may affect also its bioactivity. In this work, dextrin and periodate-oxidized dextrin (ODEX), both irradiated and non-irradiated, were hydrolysed by α-amylase and the obtained oligosaccharides were fractionated by ligand-exchange/size exclusion chromatography and analyzed by matrix-assisted laser desorption/ionization (MALDI-MS) and electrospray ionization (ESI-MSn) mass spectrometry. This allowed the identification of structures formed during partial periodate oxidation, which displayed aldehyde gro ...

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