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17α-ethinylestradiol analysis of endo- and exometabolome of Ulva lactuca (chlorophyta) by 1H-NMR spectroscopy and bioinformatics tools

  • Cabral, Debora; Cardoso, Sara; Rocco, Silvana; Sforça, Maurício; Hengeltraub, Simone Fanan; Bauer, Cláudia; Rocha, Miguel; Maraschin, Marcelo
  • Advances in Intelligent Systems and Computing, 803 (PACBB 2018), 216-223, 2019

Publisher: Springer

Abstract

The uptake of 17α-ethinylestradiol (EE2) by the green alga Ulva lactuca and its effects on the metabolome of that macroalgae have been investigated using 1H-NMR spectroscopy. To this end, U. lactuca in vitro cultures have been acutely (48 h) exposed to the estrogen and both, endo- and exometabolome were investigated. Bioinformatic analyses were performed to integrate and analyze endo- and exometabolic profiles allowing the detection of EE2 into exometabolome samples and identifying very important parameters (VIPs), i.e., 1H-NMR resonances, responsible for grouping similar U. lactuca metabolic ...

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