Publications in International Journals / Series (Article (International))

One-step chromatographic method to purify α-lactalbumin from whey for nanotube synthesis purposes

  • Food Chemistry, 275, 480-488, 2019

Publisher: Elsevier


A one-step anion-exchange chromatography method (NaCl gradient elution on a DEAE Sepharose™ Fast Flow gel column) was developed to purify α-lactalbumin (α-LA) from whey protein isolate. α-LA nearly 100% pure (based on the total protein content) was obtained with a yield of about 39%. Besides pure α-LA, which was the main objective of this work, highly pure β-lactoglobulin was also obtained with a yield of about 59%. The high purity of the obtained α-LA samples allowed its use to synthesise protein nanotubes with excellent gelation properties for their use as food thickeners and bioactive carri ...

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