MSc Dissertations

Innovative Ochratoxin A (OTA) extraction platforms using OTA-binding recombinant proteins

  • Mestrado em Biotecnologia, 2018

Abstract

Wine is a widely consumed product that is often associated with contaminations of toxic metabolites called mycotoxins. The most important mycotoxin associated to wine is ochratoxin A (OTA), and its detection usually involves the clean-up of samples with immunoaffinity columns (IAC) and quantification by HPLC coupled with fluorescence detection (FL). However, the several drawbacks associated with the use of the IAC led to the search for alternative clean-up methods. Thus, in this work, new platforms for OTA clean-up from wine were developed, based on proteins whose affinity towards OTA makes th ...

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