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Specific detection of viable Salmonella Enteritidis by phage amplification combined with qPCR (PAA-qPCR) in spiked chicken meat samples

  • Garrido-Maestu, Alejandro; Fuciños, Pablo; Azinheiro, Sarah; Carvalho, Carla M.; Carvalho, Joana; Prado, Marta
  • Food Control, 99, 79-83, 2019

Publisher: Elsevier

Abstract

Serovar Enteritidis represents 45.7% of all Salmonella reported human cases identified in Europe. Additionally, “minced meat and meat preparations from poultry” have a high level of non-compliance, regarding Salmonella regulation. In the current study, a novel method based on the amplification of the Salmonella bacteriophage vB_SenS_PVP-SE2, coupled with real-time PCR (qPCR), was developed and evaluated, for the rapid detection of viable Salmonella Enteritidis in chicken samples. The results obtained indicated that the qPCR method could detect down to 0.22 fg/μL of pure virus DNA and a concent ...

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