Publications in International Journals / Series (Article (International))

Catalytic activation of esterases by PEGylation for polyester synthesis

  • ChemCatChem, 11(10), 2490-2499, 2019

Publisher: Wiley-Blackwell

Abstract

In this work we explored PEGylation as an efficient strategy to improve esterase’s catalytic performance. For this, we PEGylated three esterases, namely lipase from Candida antarctica B (CALB), lipase from Thermomyces lanuginosus (TL) and cutinase from Fusarium solani pisi (CUT) and evaluated their catalytic performance by using the biosynthesis of poly(ethylene glutarate) as model reaction. After PEGylation with a 5 kDa aldehyde‐PEG, CALB and cutinase revealed an increase of activity against p‐nitrophenyl butyrate hydrolysis (2‐fold of increase for CALB and 4‐fold of increase for cutinase). U ...

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