Publications

Publications in International Journals / Series (Article (International))

Ohmic heating as a new tool for protein scaffold engineering

Rodrigues, Rui M.; Pereira, Ricardo N.; Vicente, António A.; Cavaco-Paulo, Artur; Ribeiro, Artur

Materials Science and Engineering C-Materials for Biological Applications, 120(111784), 2021

Publisher: Elsevier

Abstract

Ohmic heating (OH) is recognised as an emerging processing technology which recently is gaining increasing attention due to its ability to induce and control protein functionality. In this study, OH was used for the first time in the production of scaffolds for tissue engineering. BSA/casein solutions were processed by OH, promoting protein denaturation and aggregation, followed by cold-gelation through the addition of Ca2+. The formation of stable scaffolds was mostly dependent on the temperature and treatment time during OH processing. The variations of the electric field (EF) induced change ...