DeYeast Library

Project Title
Designer yeast strain library optimized for metabolic engineering applications
Project Type
Internacional / Public
Funding Body
Funding Program
ERA-IB
Reference
ERA-IB-2/0003/2013
Funding
  • CEB: 99 600,00
  • Total: 1 545 248,00
Start
01-03-2014
End
31-12-2017
Partnership
University of Minho, EMBL - European Molecular Biology Laboratory, Germany, DTU - Technical University of Denmark, University of Frankfurt, Germany, SilicoLife, Lda
External link

Principal Investigator

Team Members - CEB

Fellowship Members - CEB

Abstract

Saccharomyces cerevisiae is one of the most widely used cell factories in industrial biotechnology. However, the development of optimized yeast strains for the production of novel compounds is a time-consuming process and represents a significant cost/time burden. Key underlying reasons include the unavailability of suitable chassis cells and lack of proven modelling tools. DeYeastLibrary project aims at contributing towards overcoming these limitations by using cutting-edge computational and experimental tools. We will build upon the fact that, despite the large chemical diversity of desired biochemical products, the number of cellular pre-cursor metabolites and co-factors needed for their synthesis is relatively small. We will systematically exploit this modular design principle of nature and construct a pre-optimized yeast chassis cell library. By using this modularity concept, we will develop high-producing cell factories for 4 flagship products, viz., vanillin, poly-hydroxybutyrate (PHB), n-butanol and succinate, for which the consortium already possesses producing strains. The project will achieve its goals by bringing together leading experts in yeast genetics, metabolic engineering and mathematical modelling.