FOSYNBIO

Project Title
Synthetic biology approaches to design and construct microbial cell factories for the production of fructooligosaccharides
Project Type
Nacional / Public
Funding Body
Funding Program
02/SAICT/2017 - Projetos de Investigação Científica e Desenvolvimento Tecnológico (IC&DT)
Reference
POCI-01-0145-FEDER-029549
Funding
  • CEB: 239 908,00
  • Total: 239 908,00
Start
15-07-2018
End
14-07-2021
Partnership
UMinho

Principal Investigator

Team Members - CEB

Fellowship Members - CEB

Abstract

Fructooligosaccharides (FOS) are among the most promising prebiotics in the market. Produced from sucrose using fructosyltransferase enzymes, the process exhibits low purity and conversion yields due to large amounts of glucose released during FOS production. This leads to the presence of non-prebiotic sugars in the final product. A faster and sustainable process is herein proposed by using the whole cells of a FOS producer, which also remove other sugars, resulting in high fermentation yields and purer FOS. Zymomonas mobilis is an excellent host as it contains native enzymes able to convert glucose and fructose into other sub-products, besides being unable to hydrolyze FOS. Expressing FTases from other sources in this chassis is expected to increase the FOS yield and quality. This novel FOS producer will be engineered using synthetic biology strategies. Bioprocess optimization, scale-up and sustainability (use of non-conventional substrates) will be pursed.