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Electrochemical aptasensor array for multiple detection of human osteopontin

  • Portugaliae Electrochimica Acta, 36(1), 1-9, 2018

Publisher: Portuguese Electrochemical Society

Abstract

Electrochemical aptasensor arrays have been recently used to detect several proteins reported as disease biomarkers, mainly due to their known advantages, that include high affinity, sensitivity, specificity and low costs, among others. This study describes the development of a label-free electrochemical multi-aptasensor array, for the simultaneous detection of human osteopontin (OPN), using two specific aptamers. To enable multiplexed protein assay, RNA and DNA aptamers were immobilized in the dual-screen-printed gold working electrodes via streptavidin-biotin interaction, and using the [Fe(C ...

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