Publications in International Journals / Series (Article (International))

Downscale fermentation for xylooligosaccharides production by recombinant Bacillus subtilis 3610

  • Carbohydrate Polymers, (In Press), 2018

Publisher: Elsevier

Abstract

The global demand of prebiotics such as xylooligosaccharides (XOS) has been growing over the years, motivating the search for different production processes with increased efficiency. In this study, a cloned Bacillus subtilis 3610, containing the xylanase gene xyn2 of Trichoderma reesei coupled with an endogenous secretion tag, was selected for XOS production through direct fermentation of beechwood xylan. A mixture of XOS with a degree of polymerization ranging from 4-6 was obtained, presenting high stability after a static in vitro digestion (98.5 ± 0.2%). The maximum production yield expres ...

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