Publications in International Journals / Series (Article (International))

Optimization of an automatic counting system for the quantification of Staphylococcus epidermidis cells in biofilms

  • Freitas, Ana Isabel; Vasconcelos, Carlos; Vilanova, Manuel; Cerca, Nuno
  • Journal of Basic Microbiology, 54(7), 750-757, 2014

Publisher: John Wiley & Sons Ltd.

Abstract

Biofilm formation is recognized as the main virulence factor in a variety of chronic infections. In vitro evaluation of biofilm formation is often achieved by quantification of viable or total cells. However, these methods depend on biofilm disruption, which is often achieved by vortexing or sonication. In this study, we investigated the effects of sonication on the elimination of Staphylococcus epidermidis cell clusters from biofilms grown over time, and quantification was performed by three distinct analytical techniques. Even when a higher number of sonication cycles was used, some stable c ...

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