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Effect of sodium chloride on solute–solvent interactions in aqueous polyethylene glycol–sodium sulfate two-phase systems
- Silva, Nuno R. da; Ferreira, Luisa A.; Madeira, Pedro P.; Teixeira, José A.; Uversky, Vladimir N.; Zaslavsky, Boris Y.
- Journal of Chromatography A, 1425, 51-61, 2015
Publisher: Elsevier
Abstract
Partition behavior of eight small organic compounds and six proteins was examined in poly(ethylene glycol)-8000–sodium sulfate aqueous two-phase systems containing 0.215 M NaCl and 0.5 M osmolyte (sorbitol, sucrose, TMAO) and poly(ethylene glycol)-10000–sodium sulfate–0.215 M NaCl system, all in 0.01 M sodium phosphate buffer, pH 6.8. The differences between the solvent properties of the coexisting phases (solvent dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) were characterized with solvatochromic dyes using the solvatochromic comparison method. D ...
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