Publications in International Journals / Series (Article (International))
Trypsin purification using magnetic particles of azocasein-iron composite
- Alves, Maria Helena M.E.; Nascimento, Gabriela Ayres; Cabrera, Mariana Paola; Silvério, Sara C.; Nobre, Clarisse; Teixeira, José A.; Júnior, Luiz Bezerra de Carvalho
- Food Chemistry, 226, 75-78, 2017
Publisher: Elsevier
Abstract
This work presents an inexpensive, simple and fast procedure to purify trypsin based on affinity binding with ferromagnetic particles of azocasein composite (mAzo). Crude extract was obtained from intestines of fish Nile tilapia (Oreochromis niloticus) homogenized in buffer (01 g tissue/ml). This extract was exposed to 100mg of mAzo and washed to remove unbound proteins by magnetic field. Trypsin was leached off under high ionic strength (3 M NaCl). Preparation was achieved containing specific activity about 60 times higher than that of the crude extract. SDS-PAGE showed that the purified prot ...
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