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Characterization of levan produced by a Paenibacillus sp. isolated from Brazilian crude oil
- Mendonça, Carlos M.N.; Oliveira, Rodrigo C.; Freire, Rominne K.B.; Piazentin, Anna C.M.; Pereira, Wellison A.; Gudiña, Eduardo J.; Evtuguin, Dmitry V.; Converti, Attilio; Santos, João H.P.M.; Nunes, Cláudia; Rodrigues, Lígia R.; Oliveira, Ricardo P.S.
- International Journal of Biological Macromolecules, 186, 788-799, 2021
Publisher: Elsevier
Abstract
A levan-type fructooligosaccharide was produced by a Paenibacillus strain isolated from Brazilian crude oil, the purity of which was 98.5% after precipitation with ethanol and dialysis. Characterization by FTIR, NMR spectroscopy, GC-FID and ESI-MS revealed that it is a mixture of linear β(2 → 6) fructosyl polymers with average degree of polymerization (DP) of 18 and branching ratio of 20. Morphological structure and physicochemical properties were investigated to assess levan microstructure, degradation temperature and thermomechanical features. Thermal Gravimetric Analysis highlighted degrada ...
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