Publications in International Journals / Series (Article (International))
Exploiting the sequence of naturally occurring elastin: Construction, production and characterization of a recombinant thermoplastic protein-based polymer
- Machado, R.; Ribeiro, Artur; Padrão, Jorge; Silva, D.; Nobre, A.; Teixeira, José A.; Arias, F.J.; Cunha, A.M.; Rodríguez-Cabello, J.C.; Casal, M.
- Journal of Nano Research, 6, 133-145, 2010
Publisher: Trans Tech Publications Ltd.
Abstract
Genetic engineering was used to produce an elastin-like polymer (ELP) with precise amino acid composition, sequence and length, resulting in the absolute control of MW and stereochemistry. A synthetic monomer DNA sequence encoding for (VPAVG)20, was used to build a library of concatemer genes with precise control on sequence and size. The higher molecular weight polymer with 220 repeats of VPAVG was biologically produced in Escherichia coli and purified by hot and cold centrifugation cycles, based on the reversible inverse temperature transition property of ELPs. The use of low cost carbon sou ...
CEB Authors
Groups
Citations
Web of Science® Citations: 10
Tools
Share Content
CEB is supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding UIDB/04469/2020.
2025 © CEB - Centre of Biological Engineering. All Rights Reserved.
